St. Joseph’s College for Women (A),
Visakhapatnam
Department
Of Biochemistry With Department Of Biotechnology & Internal Quality
Assurance Cell (IQAC)
2
Day National Workshop on Immunoassays In collaboration with United Scientific
Chemicals, Visakhapatnam
3rd
to 4th September, 2021 Time:
9 AM to 4 PM
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REGISTRATION STARTS 8.45 AM AT BIOTECH/BIOCHEM LAB
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Inaugural session- Room No- T4
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9.30
am
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Welcoming
the gathering and inviting guests on to the dais
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Dr
Mousami Shankar A
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9.35 am
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Prayer song
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2nd BBC team
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9.40
am
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Rationale
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Dr
P. Mary Anupama
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9.45 am
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About College and departments
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Dr A. Veerabrahmam
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9.50
am
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Introduction
to Chief guest- Dr P. Subhashini Devi, HOD, Dept of Biochemistry, Andhra
University.
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Dr
Mousami Shankar A
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9.55 am
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Chief guest speech- Insights into
structure of Immunoglobulin genes and antibody diversity
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Dr P. Subhashini Devi
Chief guest and resource speaker
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10.45
am
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Q
and A session- Moderator
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Dr
A Veerabrahmam
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10.50 am
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Felicitation to the chief guest
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By organizers and dignitaries
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10.55
am
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Vote
of thanks
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Dr
P. Mary Anupama
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Tea break
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11.05
am
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Snacks
will be served near Biotechnology lab, First floor of main building.
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First demonstration session
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11.45
am
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Introduction
to teaching kit- Double immunodiffusion
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Dr
P. Mary Anupama
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Lunch Break From 12.25 Pm
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1.00
pm
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Demonstration
will be started for experiment 1
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Dr
P. Mary Anupama
Dr
A. Veerabrahmam
Dr
Mousami Shankar A
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3.30 pm
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SPOT EVENT- 1
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Student volunteers
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4.0
pm
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Close
of first day program
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DAY 2
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9.15 am
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Introduction to teaching kit- Radial
Immunoassay
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Dr Mousami Shankar Addala
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9.30
am
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Demonstration
of kit
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Dr
Mousami Shankar A
Dr
P. Mary Anupama
Dr
A. Veerabrahmam
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10.30 am
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Tea break
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10.45
am
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Demonstration
of experiment 2 – continued
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12.15 pm
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Lunch Break and spot event- 2
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12.50
pm
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Introduction
to Teaching kit 3- DOT-ELISA
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Dr
A. Veerabrahmam
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1.15 pm
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Demonstration of experiment – 3
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Dr A. Veerabrahmam
Dr Mousami Shankar A
Dr P. Mary Anupama
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2.30
pm
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Tea
Break
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2.40 pm
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Experiment continues
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VALIDICTORY FUNCTION
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3.30 pm
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Inviting guests of honour on to the
dias
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Dr Mousami Shanker A
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3.35
pm
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Introduction
to Dr Manikya Kumari, HOD, Dept. of Botany
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Dr
Mousami Shankar A
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3.40 pm
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Short speech by Dr Manikya Kumari mam
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3.50
pm
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Introduction
to Vice- Principal
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Dr
A. Veerabrahmam
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3.55pm
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Addressing the gathering
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Sr Hema
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4.05
pm
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Report
on Workshop
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Dr
P. Mary Anupama
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4.10 pm
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Distribution of certificates
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Dr Mousami Shankar A,
Dr A Veerabrahmam
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4.25
pm
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Feedback
by participants
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4.30 pm
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Vote of thanks
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Dr P. Mary Anupama
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4.35pm
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National
anthem
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2nd
BBC students
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Rationale for National Workshop on
Immunoassays
Dates- 3rd and 4th August, 2022
Honorable chief guest and
resource speaker for today’s program- Prof.P. Subhashini Devi mam, respected
heads of the departments and faculties of st. joseph’s college for women and
invited Heads of the departments from various colleges who came to be a part of
the workshop, dear students and participants of the workshop.
This is Dr P. Mary
Anupama, Coordinator for the workshop and head of the department of Biochemistry
of SJC extended a warm welcome to the 2 day National workshop on Immunoassays
which is being organized jointly by the Departments of Biochemistry and
biotechnology of SJC in collaboration with United Scientific chemicals, Vsp.
This is our first offline
workshop after covid.
We have seen a immense
progression in assaying antigens and antibodies during covid time. We assay
viral load and antibody levels in our body using RT PCR and rapid test. While
some basic antigen -antibody analysis methods remain the same for decades.
These are used as the simplest inexpensive methods and have played very
important role in various bioanalytical settings, such as clinical diagnostics,
biopharmaceutical analysis, environmental monitoring, security to food testing.
We have both qualitative and quantitative methods that have evolved over the
years that detect even the minute quantities of antigens.
Tremendous advances in bioanalytical platforms, immunoanalytical
systems, and complementary technologies have led to various emerging Immunoassay
formats.
Initially, most of the IAs, developed several decades ago, were
based on radioimmunoassay (RIA) and enzyme-linked immunosorbent assay (ELISA).
Consequently, RIA- and ELISA-based kits evolved for a wide range of analytes and
these became commercially available as the antibodies were generated against
such analytes.
Owing to extremely high sensitivity, specificity, precision, and
throughput of ELISA, this format has served as the gold standard for a plethora
of analytes. The last two decades has seen tremendous innovation in ELISA
technology.
Through this workshop we will be demonstrating 3 teaching kits-
The Ouchterlony method, Radial Immunoassay and Dot – ELISA.
We seek you cooperation as we proceed to the practical sessions
after inaugural session.
Thank you all once again for honoring our invitation and being a
part of it.
As the antigens interact with antibodies during the course of
workshop, we wish to know more about our participants during the next 2 days of
stay. have a wonderful time at SJC. Thank you.
Report:
First day of workshop- 3rd August , 2022
The inaugural function was joined by chief guest Prof.
P. Subhashini Devi, Prof. PK Jayalakshmi, Dr Manikya Kumari, Dr Harini, Ms.
Ramani Dhanaraj, Dr Padma Sundari, Dr Ramesh, Dr Praveen, Dr Rosina George, Dr
Neha, Ms Indrani, Ms Prameela, Ms. Veena, Vicky Hale mam and many other faculty
of the college.
Overall 8 colleges for registered for the event-
Andhra Unviersity, Bullaya college, VS krishna College, AVN college, Gayatri
vishya Prarishad, St. Anns College and students of PG from Home science
department, St. Joseph’s college college, along the host department students of
2nd BBC.
Total students
registered- 118 + 5 faculty from various colleges
The chief guest has given her lecture on Antibody
diversity which covered about introduction to immune system, the cells and
organs of immune systems. Madam discussed about the cell proliferation and the
changes that take place in specific in the B cell. As the cells mature, the
cells express initially IgM and then IgD. These cells express other types of
antibodies due to the class switching in the genes. The lecture included the events
taking place at the germinal center of a secondary follicles in the secondary
lymphoid organs. The various genes that give the antibody diversity at
Hypervariable region include that which code for light chain and the variable chain
variable regions. These lead to antibody diversity which account ot more than 4
lakhs types of antibodies. The diversity with respect to TCR was also discussed
and was compared to antibody diversity.
This was followed by first practical demonstration
session for the kit- Ouchterlony method.
The method was explained was Dr P. Mary Anupama and
was demonstrated by Dr Anupama, Dr Veerabrahmam and Dr Mousami for all the
students. They prepared the agarose slides and loaded antigens and antibodies
and saved the slides to view next day.
Report:
Second day of workshop- 4th August, 2022
The 2nd day workshop started with
Explanation of the 2nd kit- Radial immunoassay by Dr Mousami Shankar
. Experiment was demonstrated by the 3 coordinators. Spot event was conducted
in between during the breaks.
The 3rd kit was explained was Dr A. Veerabrahmam
and was demonstrated by all the 3 coordinators.
Valedictory function was graced by Chief guest, Sister
Hema who is the Vice – principal of SJCW and Guest of Honor Dr Manikya Kumari
mam who is the Head of the department of Botany. Dr Manikya kumari mam
explained the importance of the study of immune system, analysis methods and
illustrated the need to have good health and ways to get good health. Sister Hema
explained how one can be active and maintain good immunity. She gave tips on
good health.
This was followed by feedback from participants, where
many of them expressed their happiness to be a part of this hands on training
workshop
Certificate distribution was done and the event
closed with group photographs.
PICTURE PROOFS
INAUGURAL SESSION AND
LECTURE BY PROF. SUBHASHINI MAM
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